Drought Stress Study on Nicotiana tabacum L., “Baladi”, an In Vitro Experimental Model

Crops drought tolerance is a trait of outmost importance for agriculture especially today when climate change affecting more the production food and feed. The scope this article to evaluate in vitro stress response Nicotiana tabacum L., “Baladi”. experiment was set up four successive stages starting with seedling development, hypocotyl cultivation, three generations micropropagation, pre-acclimatization acclimatization. effect abscisic acid (ABA) and/or polyethylene-glycol 6000 (PEG) on tobacco caulogenesis micropropagation were investigated. Superoxide-dismutases (SODs) peroxidases (POXs) are active different isoforms patterns have been identified compared control cualogenesis. A decrease internodes length higher shoots multiplication rate observed. However, under PEG treatment plantlets expressed hyperhydration ceased rooting. Pre-treatments effects study ABA finalized acclimatization phase 18 clones. summary our results revealed that induce among others oxidative first stage not maintained all clones until Certain lower SOD activity during but maintaining POX activity.